Biophysics of type IV pili assembly

Published
WorkplaceParis, Ile-de-France, France
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Position


biophysics-of-type-iv-­pili-assembly/" EN

biophysics-of-type-iv-­pili-assembly/" FR

Revenir Haut de page Postdoc position to study recombination among enteroviruses Human microvessel (red) colonized by N. meningitidis (green).

Emploi

Biophysics of type IV pili assembly

Équipe: Pathogenèse des infections vasculaires

Projet transversal: Biologie quantitative

Membre : Guillaume Dumenil

Domaines Scientifiques

Maladies

Organismes

Applications

Technique

30

Jun 2024

Postdoctoral position Biophysics of type IV pili assembly Research Project Type IV pili machineries are bacterial molecular nanomachines found in numerous pathogenic bacteria that allow the dynamic assembly of a polymeric fiber composed of [...]

Postdoctoral position

Biophysics of type IV pili assembly Research Project Type IV pili machineries are bacterial molecular nanomachines found in numerous pathogenic bacteria that allow the dynamic assembly of a polymeric fiber composed of one protein, the major pilin. These fibers play essential roles in bacterial physiology and pathogenesis. Recent approaches allowing the visualization of type IV in real time revealed that these filamentous structures form by the assembly of a staggering 1000 monomers per second and can disassemble at the same speed. A physical understanding of these extremely fast processes, which combine passive diffusion of monomers and their active, motor driven assembly/dissaembly, is still missing. Fluorescence microscopy including single molecule approaches will provide the basis for a full description of the process at the biophysical level. Theoritical aspects will be explored in the context of a collaboration with Raphael Voituriez (Laboratoire Jean Perrin, CNRS - Sorbonne Université).

Host lab

This project will be funded by an ERC AdG at the Pathogenesis of Vascular Infections Unit (Head: Guillaume Duménil) at Institut Pasteur (Paris, France). The candidate will be integrated in an interdisciplinary team that combines biochemistry, microbiology, cell biology, biophysics and animal models of infection. This project will take advantage of the vivid scientific environment of the campus including in quantitative biology and the numerous available core facilities.

Activities The successful candidate will participate in innovative and integrated approaches to understand the function of protein nanomachines. This includes sample preparation, fluorescence microscopy, image analysis and modelling. All these expertises are present in the host lab and reinforced by well-established collaborations.

Knowledge and skills We are looking for a motivated young scientist with a PhD in biophysics or phyics with interest in biological questions. Experience in fluorescence based imaging is expected. Programming skills is recommended. The candidate should feel comfortable with interdisciplinary science. Excellent communicational and presentational skills in English is expected.

Artistic representation of a bacterial aggregate of Neisseria meningitidis aspirated in a micropipette
In your application, please refer to myScience.fr and reference JobID 44373.