- Cell nuclei protect the DNA. Nuclei can rupture when cells are deformed, causing DNA damage.
- In the case of breast cancer, this damage makes tumour cells more invasive, with increased risk of metastasis.
When cells multiply and migrate, they can be compressed and their nucleus may break open. This phenomenon causes DNA damage. Scientists from the CNRS, Institut Curie and INSERM have now shown that this facilitates the spread of cancer cells in breast tumours. The results of this research were published on 21 September in Cell.
The nucleus of a cell fills a crucial function: to protect cell DNA and enable its proper use. But it can be deformed, or even temporarily fractured, if the cell itself is compressed and deformed, for example, in the case of migration or proliferation. This compression then leads to DNA damage. The consequences are accelerated ageing for healthy cells and the acquisition of invasive properties for breast tumour cells, as has just been shown by a research team from CNRS, Institut Curie and INSERM 1 .
These scientists have shown that when cells are compressed and that breaks the nucleus, DNA can come into contact with a DNA-destroying enzyme called TREX1. TREX1’s normal function is to protect the cell by destroying the DNA of viruses that try to infect it, but under these unusual conditions it attacks the cell DNA.
In healthy tissue, the cells then show signs of ageing and stop dividing. However, the research team has observed that the consequences are different in a breast tumour: instead of killing the cancer cells, the damage caused by TREX1 will make them more invasive. For example, when the tumour grows too much, the cells are compressed and then acquire the ability to destroy their environment to invade neighbouring tissues, with increased risk of metastasis.
These results reveal the importance of the enzyme TREX1 in the development of breast cancer and also in ageing. The scientists now want to identify and test molecules that could block its activity. Because TREX1 has an important role in modulating inflammation and immunity, these inhibitors may have many applications in therapy.
- 1. In France, researchers from the Laboratoire Biologie Cellulaire et Cancer (CNRS/Institut Curie/Sorbonne Université), the Laboratoire Immunité et Cancer (INSERM/Institut Curie), the Laboratoire Physico-chimie Curie (CNRS/Sorbonne Université/Institut Curie), the Laboratoire Processus d’Activation Sélectif par Transfert d’Energie Uni-électronique ou Radiatif (CNRS/ENS - PSL/Sorbonne Université) and the Centre de Recherche des Cordeliers (INSERM/Université de Paris/Sorbonne Université) participated in this work.
Compromised nuclear envelope 1 integrity drives TREX1-dependent DNA damage and tumor cell invasion. Guilherme Pedreira de Freitas Nader, Sonia Agüera-Gonzalez, Fiona Routet, Matthieu Gratia, Mathieu Maurin, Valeria Cancila, Clotilde Cadart, Andrea Palamidessi, Rodrigo Nalio Ramos, Mabel San Roman, Matteo Gentili, Ayako Yamada, Alice Williart, Catalina Lodillinsky, Emilie Lagoutte, Catherine Villard, Jean-Louis Viovy, Claudio Tripodo, Jérôme Galon, Giorgio Scita, Nicolas Manel3, Philippe Chavrier and Matthieu Piel. Cell, 21 September 2021. DOI:10.1016/j.cell.2021.08.035